Nederlandse Vereniging voor Klinische Chemie en Laboratoriumgeneeskunde
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Onderzoekslijn 23 Tissue Engineering
Trefwoord 1
Trefwoord 2
Omschrijving A scaffold for tissue engineering should initially act as an adhesive substrate and a physical support for cells. During proliferation of the cells and production of extra-cellular matrix, the scaffold should degrade leaving in its place a new patch of tissue. Furthermore, the mechanical properties of the scaffolding material should match those of the surrounding tissue to allow transmission of mechanical stimuli to the cells in order to develop the required phenotype and orientation. Trimethylene carbonate (TMC) based polymers are flexible polymers and degrade slowly by hydrolysis in vitro and more rapidly in vivo by surface erosion. Lactide and glycolide (co)polymers, which are most often used in tissue engineering, do not possess these advantageous properties.
A suitable environment for tissue generation can be created by incorporating biologically active factors such as growth factors and differentiation factors in the scaffold. As succesful vascularization of the tissue engineered structure is essential for nutrient transport and integration, such biological activity should also be directed at promoting angiogenesis. TMC-based polymers are most suitable for the controlled delivery of proteins and growth- and differentiation factors as acidic compounds are not generated upon degradation of the polymer and denaturation or loss of activity can be avoided. Preliminary research performed in our group has shown that it is possible to prepare nanospheres and microspheres from TMC based polymers, and also thermally reversible hydrogels from PEG-PTMC block copolymers could be prepared. TMC is a component in surgical sutures, approved and clinically applied. These properties, make TMC-based polymers very useful in tissue engineering.

In this project, several aspects of the prepartion, evaluation and application of tissue engineering scaffolding structures will be investigated:
- thermoreversible gels incroporating growth factor releasing microparticles will be prepared and evaluated for their cpability of inducing angiogenesis in vitro and in vivo
- injectable hydrogel systems in which heparin is covalenty bound will be developed for the delivery of stem cells and their differentiation into cartilage or bone tissue via the release of heparin-binding growth factors
- biomimetic hybrid structures based on thermosensitive hydrogels and natural components of the extracellular matrix and biologically active peptide sequences will be prepared and evaluated with regard to their chemical, physical and biological properties.
- cardiac tissue will be grown from neo natal rat heart cells incorporated in flexible and elastic porous structures and evaluated in vivo
- human mesenchymal stem cells will be embedded in elastic porous tubular structures and differentiated into smooth muscle cells and endothelial cells for the generation of small-caliber blood vessel equivalents
Onderzoeksleider laboratorium Prof.dr. I.(Istvan) Vermes
Mede-onderzoeksleiders Prof. Dr. J. Feijen, University of Twente
Instituut Medlon BV Enschede
Periode 1-2001 - 1-2009