Nederlandse Vereniging voor Klinische Chemie en Laboratoriumgeneeskunde
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Onderzoekslijn 20 Lab-on-a-Chip
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Omschrijving It is known that a single differentiated eukaryotic cell performs 103-104 different chemical operations simultaneously in an extremely small volume at overall burning rate of 106 molecules of ATP. All this is performed in a volume element in the order of 1 picoliter. How is this chemical multiprocessing capability in extremely small volumes possible. The response of cells to extracellular signals involves the activation of intracellular signal transduction pathways and induction of gene transcription in the nucleus. Intracellular signal transduction involves an intricate network of signal transduction proteins present between plasmamembrane and nucleus that acts by protein modification and protein-protein association. Abnormal activation or mutations in genes encoding signal transduction proteins has been demonstrated to be responsible for a large variety of human pathological conditions. Up to now, these complex processes have not been easily accessible at the single cell level. One of the future challenges therefore is to analyze the state of activation of signal transduction within a isolated cell. Within the LIC (Lab-in-a-Cell) concept this can be achieved by reproducible analysis of an extremely small quantity cellular content with regard protein conformation, protein binding of co-factors, state of phosphorylation and presence of proteins in different subcellular compartments. If these goals can be achieved, the LIC concept will be extremely useful for clinical diagnosis and therapy for instance in monitoring the effects of drugs. Also the successful isolation of cells (e.g. stem cells) from mixed populations by LIC is envisioned. The aim of this session is to combine the expertises of micro/nanotechnology and clinical biochemistry in order to exploit opportunities for clinical diagnostics.
Onderzoeksleider laboratorium Prof.dr. I.(Istvan) Vermes
Mede-onderzoeksleiders Prof. dr. ir. A. van den Berg, BIOS The Lab-on-a-Chip group, MESA+ research institute University of Twente Prof. dr. H. Andersson, Royal Institute of Technology, Dept.Signals, Sensors & Systems, Stockholm, Sweden
Instituut Medlon BV Enschede
Periode 1-2002 - 1-2011