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|Onderzoekslijnen||Alle projecten||Alle publicaties en voordrachten||Instituten|
|Project 53||Tissue engineering of small-caliber blood vessels|
|Omschrijving||Mesenchymal stem cells from bone marrow have been shown to undergo de-differentiation when cultured on fibronectin in the presence of PDGF-BB as well as a number of other mitogens and co-factors. Forward differentiation of these de-differentiated cells into vascular smooth muscle and endothelial cells has been demonstrated. This indicates that a common precursor for vascular smooth muscle and endothelial cells can be generated from bone marrow-derived mesenchymal stem cells.
Human mesenchymal stem cells (CD34-, CD45-; CD29+, CD44+, CD90+, commercially available from Orisis or from single bone marrow donors) will be cultured on fibronectin in the presence of PDGF-BB. During culture, the expression of Oct-4 and Flk1 will be investigated by semi-quantitative PCR and immunofluorescence and used as markers for (increased) pluripotency and VEGF responsiveness, respectively. Differentiation in vascular endothelial cells will be induced by transferring the cells to collagen type IV as substrate followed by addition of VEGF (20 ng/ml). Differentiated derivatives will be investigated for expression of markers characteristic of vascular endothelial cells including von Willebrand Factor, PECAM1 and E and P selectin. Differentiation into vascular smooth muscle cells will be induced by plating the cells on collagen and treatment of confluent cells with PDGF-BB. Differentiation will be characterized by analysis of expression of the smooth muscle cell markers α-SMA and PDGF-β receptor.
|Projectleider||Prof.dr. I.(Istvan) Vermes|
|Instituut||Medlon BV Enschede|
|Trefwoorden||vascular grafts, stem cells, small-caliber blood vessels,|
|Periode||1-2001 - 1-2009|
|Partners||Prof. dr. J. Feijen, University of Twente
Dr. R. Geelkerken, chirurg MST
|Medewerkers||H. Weekamp, PhD student
P. Engbers-Buijtenhuijs, assistent in opleiding
dr. A.A. Poot, hoofddocent University of Twente
M. Kamphuis, research technician
|Financiering||1e Geldstroom - Intern
Dutch Program for tissue engineering (DPTE)