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PPO Database - onderzoekslijnen
||Validity of free testosterone calculation in pregnant women
||Klinisch b. Endocrinologie en intermediaire stofwisseling
||Objective: Increased maternal testosterone concentration during pregnancy may affect the fetus. Therefore it is clinically relevant to have a quick and reliable method to determine free testosterone levels. Current calculators for free testosterone are suspected to perform poorly during pregnancy due to suggested competition between high levels of estradiol and free (bio-active) testosterone for sex hormone-binding globulin (SHBG) binding. Therefore, it is claimed that reliable calculation of free testosterone concentration is not possible. However, recent evidence on SHBG binding sites questions the estradiol effect on the testosterone-SHBG binding during pregnancy. In this study, we investigated whether the free testosterone concentration can be calculated in pregnant women.
Design and Methods: Free testosterone was measured with a specially developed equilibrium dialysis method combined with liquid chromatography tandem mass spectrometry (LC-MS/MS). Free testosterone was also calculated with the formulas of Vermeulen et al. and Ross et al.
||Dr. M.P.(Martin) Schuijt
||C.G.J. Sweep , R. van der Steen, A.J. Olthaarb, N.M.M.L. Stikkelbroeck, H.A. Ross, A.E. van Herwaarden
||11-2015 - 7-2018
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